Vitrification as an Alternative Approach for Sperm Cryopreservation in Marine Fishes

Cryopreservation of sperm in marine fish

Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evalua...

Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols.

OBJECTIVE To study the thermal properties of a cryoprotectant solution, called VS4, and of VS4-impregnated whole sheep ovaries with pedicle. DESIGN Physical and experimental animal study. SETTING Academic research environment. ANIMAL(S) Five- to 6-month-old ewes. INTERVENTION(S) Thermal properties on cooling of a cryoprotectant solution called VS4 were measured by differential scanning ...

Large volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa.

Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free vitrification to conventional cryopre...

Does natural honey act as an alternative to antibiotics in the semen extender for cryopreservation of crossbred ram semen?

Antibiotics are added to semen extenders to take care of heavy microbial load, however, their continuous use poses a constant threat of developing antibiotic resistance by the common microbes present in the semen. Our hypothesis was that natural honey, having antibacterial activity and rich in fructose could replace the use of antibiotics and fructose in the semen extender. Twenty-four ejaculat...

Vitrification: An Emerging Technique for Cryopreservation in Assisted Reproduction Programmes